Profiling of 120 types of herbal extracts and their effects on morphology in cultured neuronal or glial cell lines, followed by <scp>RNA</scp> extraction for a <scp>cDNA</scp> library: Consideration for use in studies based on Kampo theories

Aim Kampo medicine is composed of a variety crude drugs. Each drug has its own effects, and the interaction all drugs produces therapeutic effect medicine. In order to examine characteristics drugs, 120 types herbal medicines were characterized based on their three-dimensional high-performance liquid chromatography (3D-HPLC) profiles as well effects cell morphology/proliferation when added cultured cells gene expression changes such BNIP-3 mRNA levels an example. Methods The neural line N18-TG-2 glial C6Bu-1 used in experiments. After acquisition 3D-HPLC data, each was culture medium at concentrations 10 100 ?g/mL, images taken morphological changes, RNA extracted prepare complementary DNA (cDNA) library. Results All results obtained from treatments with are listed Supporting Information. Based these example antidepressant-related factor BNIP-3, which we had previously found increase after treatment Hochuekkito, studied revealed that it increased by Astragali Radix, Cimicifugae Rhizoma, Glycyrrhizae Radix others. Conclusion present results, collected observations under unified conditions, include information will promote research. Future studies combinations also discussed, suggesting files cDNA library presented here can be basis for many future integrated new Wakan-yaku prescription developments.